- ￥5000
- GeneTex
- 美国
- GTX39796
- 2025年07月10日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Cultured bone marrow cells
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX39796
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS
- 浓度：
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
- 抗体英文名：
Ly-6B.2 antibody [7/4] (Low endotoxin, azide free)
- 抗体名：
Ly-6B.2 抗体 [7/4] (Low endotoxin, azide free)
- 规格：
100 μg
FACS analysis of mouse peripheral blood granulocytes using GTX39796 Ly-6B.2 antibody [7/4] (Low endotoxin, azide free).
IHC-Fr analysis of mouse lymph node tissue using GTX39796 Ly-6B.2 antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI.
IHC-Fr analysis of mouse lymph node tissue using GTX39796 Ly-6B.2 antibody [7/4] (Low endotoxin, azide free).
IHC-Fr analysis of mouse lymph node tissue using GTX39796 Ly-6B.2 antibody [7/4] (Low endotoxin, azide free).
IHC-Fr analysis of mouse lymph node tissue using GTX39796 Ly-6B.2 antibody, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18, red in B. C is the merged image with nuclei counterstained blue using DAPI.
IHC-Fr analysis of mouse lymph node tissue using GTX39796 Ly-6B.2 antibody [7/4] (Low endotoxin, azide free).
IHC-Fr analysis of C57BL/6 mouse spleen tissue using GTX39796 Ly-6B.2 antibody [7/4] (Low endotoxin, azide free).
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文献和实验Shu F et al., Front Immunol 2018 (PMID:29896195)
Purification of Endotoxin Free mAbs
in endotoxin free water containing 10mM EDTA and subsequently stored sterile in endotoxin free water containing 0.1% sodium azide). Finally, centrifuge (at least 3500rpm for 30 min in bench centrifuge) and 0.2 micron sterile filter to remove any grossly
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
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