Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Rat
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX109162
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, IHC-P, IP
浓度:
0.13 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
4E-BP1
抗体英文名:
4E-BP1 antibody [N1C3]
抗体名:
4E-BP1 抗体 [N1C3]
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Immunoprecipitation of 4E-BP1 protein from HCT116 whole cell extracts using 5 μg of 4E-BP1 antibody [N1C3] (GTX109162) or 4E-BP1 antibody [N1C3-2] (GTX116315). Western blot analysis was performed using 4E-BP1 antibody [N1C3] (GTX109162). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using EIF4EBP1(GTX109162) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX109162) diluted at a dilution of 1:2000.
Application Reference
Yeh YH et al., J Exp Clin Cancer Res 2018 (PMID:29587825)
Yanqi Xie et al., Mol Cancer Ther 2021 (PMID:34376582)
Wu YY et al., J Exp Clin Cancer Res 2019 (PMID:31262325)
Liu YH et al., Phytomedicine 2018 (PMID:30097117)