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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human HP1 alpha. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX113944
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
HP1 alpha
- 抗体英文名:
HP1 alpha antibody
- 抗体名:
HP1 alpha 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
HP1 alpha antibody detects HP1 alpha protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% PFA at RT for 15 min.
Green: HP1 alpha protein stained by HP1 alpha antibody (GTX113944) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa nucleus
12% SDS PAGE
GTX113944 diluted at 1:10000
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
TSA Treatment of Mammalian Cells
) towards the nuclear periphery. HP1 domain disruption upon TSA treatment is monitored by immunofluorescence using an antibody against HP1alpha (2HP1H5) from EUROMEDEX. Reversibility of the drug effect was obtained within 20 hours after TSA removal. (Figure
or HP1s associated with 5' biotin labeled siRNA EF52. Schematic methodology is shown for detection from cell lysates of biotin labeled siRNAs in complex with flag-tagged DNMTs or HP1s (alpha, beta, or gamma). B) Triple immunoprecipitation assay
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