- ￥5600
- GeneTex
- 美国
- GTX81004
- 2025年07月16日
- WB, IHC-P, FACS
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2AH.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX81004
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A type 1H
- 抗体英文名：
Histone H2A type 1H antibody, N-term
- 抗体名：
Histone H2A type 1H 抗体, N-term
- 规格：
400 μl
IHC-P analysis of human brain tissue using GTX81004 Histone H2A type 1H antibody, N-term.
WB analysis of A2058, A375, K562, HeLa, MCF-7 cell lysate (35ug/lane) using GTX81004 Histone H2A type 1H antibody, N-term.
FACS analysis of MCF-7 cells using GTX81004 Histone H2A type 1H antibody, N-term.
Green : primary antibody
Blue : negative control
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文献和实验Wang M et al., Pathol Oncol Res 2018 (PMID:29502225)
Histone Acetylation and Deacetylation
residues in the N-terminal tails of the core histone can be post-translationally modified by acetylation of the ε-amino group. The dynamic equilibrium of core histone acetylation is established and maintained by histone acetyltransferase (HAT) and histone
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
in hippocampal neurons and striatal neurons (Li et al., J Neurochem 90:1117–1131, 2004; Crosio et al., J Cell Sci 116:4905–4914, 2003). Co-staining with a cell-specific antibody will allow us to determine the type of cells that show activation of histone
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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