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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX48614
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
p38 MAPK (phospho Thr180/Tyr182)
- 抗体英文名:
p38 MAPK (phospho Thr180/Tyr182) antibody
- 抗体名:
p38 MAPK (phospho Thr180/Tyr182) 抗体
- 规格:
100 μl
Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red using p38 MAPK (phospho Thr180/Tyr182) antibody (GTX48614)
Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182 using p38 MAPK (phospho Thr180/Tyr182) antibody (GTX48614)
Western Blot: 38MAPK Phosphospecific Antibody [GTX48614] - Western blot of C-6 glioma cell lysates that had been incubated in the absence (Control) or presence of anisomycin showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.
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文献和实验Lin JT et al., Journal of Functional Foods 2018 47()
Cho EC et al., Mediators Inflamm 2015 (PMID:26089597)
Er-Chieh Cho et al., Mediators of Inflammation 2015
Tseng HL et al., Toxicol Appl Pharmacol 2012 (PMID:22842013)
Sinanoglu O et al., Urology 2012 (PMID:22951000)
Yu-Jen Chiu et al., Oncol Rep 2021 (PMID:34013378)
Chi-Cheng Lu et al., Exp Ther Med 2021 (PMID:34131445)
Chen CF et al., Oncol Lett 2018 (PMID:30333889)
Lu CC et al., Int J Oncol 2019 (PMID:30720062)
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选,选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时,又用传统的WB方式来验证这个结果。经过反复验证,都得出了同样的结果, Cell Based
kinase is KIR 3.1 and have developed a phospho-selective antibody against the Y12 motif of this channel. Much like KOR and p38 MAP kinase, phosphorylation of this potassium channel increases following repeated stress. The following chapter discusses
【求助】请问如何检测RAS、MAPK、p38、p21 ?参与者:liqunfeng请问如何检测RAS、MAPK、p38、p21??谁帮帮我啊参与者:xiaolx你在网上搜搜别人的实验中怎么检测的,不就知道了。p38,我师姐做过免疫组化。参与者:zhangyuelin1999检测信号通路现在用的比较多的方法应该是Western blot,它是测细胞中的蛋白含量(半定量)的最好方法了,免疫组化检测是定性的!如果时间充裕的话,你可以两种方法都可以做的。RAS:Ras蛋白是细胞内的一种小分子GTP结合
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