Matrices listed below were spiked with certain level of TPO-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TPO-Ab in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-105
99
EDTA plasma(n=5)
90-104
97
heparin plasma(n=5)
94-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.