Matrices listed below were spiked with certain level of β-CTx and the recovery rates were calculated by comparing the measured value to the expected amount of β-CTx in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
97
EDTA plasma(n=5)
87-105
98
heparin plasma(n=5)
88-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-CTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.