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- 文献和实验
- 技术资料
- 样本:
血清、血浆或其它相关生物液体中天然和重组β-Hex A蛋白
- 库存:
30
- 标记物:
血清/血桨/组织等
- 适应物种:
小鼠
- 应用:
用于科研使用
- 检测方法:
酶联免疫吸附测定法
- 检测范围:
0.313-20ng/ml
- 供应商:
上海信裕生物科技有限公司
- 规格:
48T/96T
| Product name | Mouse β-Hex A (β-hexosaminidase A) ELISA Kit |
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| Catalogue No. | XY-EM1459 | ||||||||||||||||||||
| Alias | β-Hex A ELISA Kit |
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| Detection method | Sandwich ELISA, Double Antibody |
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| Size | 96T |
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| Range | 0.313-20ng/ml |
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| Sensitivity | < 0.188ng/ml |
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| Standard curve | ![]() |
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| Storage | 4 ℃ for 6 months |
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| Recovery | Matrices listed below were spiked with certain level of β-Hex A/HEXA/TSD/beta-hexosaminidase subunit alpha/Beta-N-acetylhexosaminidase subunit alpha/hexosaminidase A (alpha polypeptide) /Hexosaminidase subunit A/N-acetyl-beta-glucosaminidase subunit alpha and the recovery rates were calculated by comparing the measured value to the expected amount of β-Hex A/HEXA/TSD/beta-hexosaminidase subunit alpha/Beta-N-acetylhexosaminidase subunit alpha/hexosaminidase A (alpha polypeptide) /Hexosaminidase subunit A/N-acetyl-beta-glucosaminidase subunit alpha in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-Hex A/HEXA/TSD/beta-hexosaminidase subunit alpha/Beta-N-acetylhexosaminidase subunit alpha/hexosaminidase A (alpha polypeptide) /Hexosaminidase subunit A/N-acetyl-beta-glucosaminidase subunit alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra-Assay: CV<8% |
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| Note | For Research Use Only |
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
每孔上样总蛋白量一致,实现样本间公平对比。 三、实战 protocol:肿瘤组织 IFN-γ 检测完整流程 以 “磁控 CAR-T 模拟细胞治疗后,肿瘤组织 IFN-γ 浓度检测” 为例,操作如下: 1.实验准备:试剂(abs9225、abs9161、abs9232、Mouse IFN-γ ELISA Kit 等)、仪器(组织处理器、高速冷冻离心机、酶标仪等)、治疗组与对照组小鼠肿瘤组织。 2. 组织裂解:肿瘤组织离体后用 PBS 冲洗吸干,精准称量 10mg 放入研磨管;加入 100μl 现配
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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