- ￥5600
- GeneTex
- 美国
- GTX83006
- 2025年07月11日
- WB, IHC-P, FACS, ELISA
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human ERK1/2 expressed in E. Coli.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX83006
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IHC-P, FACS, ELISA
- 靶点：
ERK1/2
- 抗体英文名：
ERK1/2 antibody [3F8]
- 抗体名：
ERK1/2 抗体 [3F8]
- 规格：
100 μl
WB analysis of Jurkat (1), HeLa (2), A431 (3) and NIH3T3 (4) cell lysate using GTX83006 ERK1/2 antibody [3F8].
FACS analysis of Jurkat cells using GTX83006 ERK1/2 antibody [3F8].
Green : ERK1/2
Purple : negative control
IHC-P analysis of human Liver tissue using GTX83006 ERK1/2 antibody [3F8].
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
per immunoprecipitation. You can use less (as little as 50) but the higher amount gives you a good, easily detectable amount of activity. 9.) To the lysate, add 1.5 μg of anti-ERK antibody (Santa Cruz, SC154 (Erk2) or SC93 (Erk1)), and incubate, with rocking
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