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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of SYK (aa296-484) expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83029
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 靶点:
Syk
- 抗体英文名:
Syk antibody [8C1A3]
- 抗体名:
Syk 抗体 [8C1A3]
- 规格:
100 μl
WB analysis of truncated SYK-His recombinant protein (1) and PMA induced THP-1 cell lysate (2) using GTX83029 Syk antibody [8C1A3].
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文献和实验General Principles of Immunoprecipitation
---------------------------------------------------------------------------- Beware of fragile kinases.The kinase activities of Syk and the p60src proteins of tsRSVs,are labile.Most of their activity is lost in RIPA.For these proteins,it is important to use NP-40 buffer,containing EDTA
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
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