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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of WNT1 expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83320
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS, ELISA
- 靶点:
Wnt1
- 抗体英文名:
Wnt1 antibody [10C8]
- 抗体名:
Wnt1 抗体 [10C8]
- 规格:
100 μl
FACS analysis of HeLa cells using GTX83320 Wnt1 antibody [10C8].
Green : Wnt1
Purple : negative control
WB analysis of NIH3T3 (1), NIH3T3L1 (2) and HeLa (3) cell lysate using GTX83320 Wnt1 antibody [10C8].
IHC-P analysis of human adrenal glands using GTX83320 Wnt1 antibody [10C8].
ICC/IF analysis of HeLa (left) and 3T3-L1 (right) cells using GTX83320 Wnt1 antibody [10C8].
Green : Wnt1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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文献和实验Vollersen N et al., Bone Res 2021 (PMID:34759273)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
consists of 5% (w/v) dried skim milk in Trisbufferedsaline/Tween-20 (TBS-T) buffer (50 mM Tris-HCl,pH 7.5, 150 mM NaCl, and 0.05% (v/v) Tween-20). 4. All antibodies are diluted 1:1,000 in 1% (w/v) dried skim milkin TBS-T, except the CRMP2 antibody (1:250
Intracellular Immunofluorescent Staining for Flow Cytometry
: Intracellular Staining Quick Guide Non-Cytokine Proteins: Intracellular Staining Quick Guide Antigen Antibody Mouse/Rat Bcl-2 10C4 Mouse CTLA-4 (CD152) UC10-4B
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