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- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human NME1 expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83325
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS, ELISA
- 靶点:
nm23-H1
- 抗体英文名:
nm23-H1 antibody [4B2]
- 抗体名:
nm23-H1 抗体 [4B2]
- 规格:
100 μl
WB analysis of nm23-H1(AA: 27-177)-hIgGFc transfected HEK293 cell lysate using GTX83325 nm23-H1 antibody [4B2].
IHC-P analysis of human Placenta tissue using GTX83325 nm23-H1 antibody [4B2].
ICC/IF analysis of HeLa cells using GTX83325 nm23-H1 antibody [4B2].
Green : nm23-H1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
FACS analysis of Jurkat cells using GTX83325 nm23-H1 antibody [4B2].
Green : nm23-H1
Purple : negative control
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文献和实验Kim D et al., J Clin Med 2020 (PMID:32977620)
nm23 Protein Expression by Western Blotting in Patients with Epithelial Ovarian Carcinoma
(4 ), they retain most of the same epitopes; thus any polyclonal antibody raised against one of the two isoforms or a monoclonal antibody against a conserved epitope will recognize both nm23-H1 and -H2 at the same time. Analysis by immunohistochemistry
Mini-Antibody Affinity Chromatography of Lysozyme
Multiple noncovalent forces do play a role in the binding between a protein antigen and an antibody directed against that protein. An antigen is bound by the antigen binding site of an antibody that consists of three hypervariable segments
s。活化的C1s依次裂解C4和C2,生成C4b2a(C3转化酶),进而裂解C3为C3a和C3b;C3b与C4b2a结合形成C5转化酶(C4b2a3b),启动膜攻击阶段。旁路途径不依赖抗体,由细菌脂多糖、真菌等病原体表面直接激活。C3在生理条件下可自发水解为C3b,在Mg²⁺存在下与B因子结合,被D因子切割形成C3bBb(旁路C3转化酶),P因子可稳定此酶。该酶大量裂解C3,形成正反馈放大环路,最终生成C3bBb3b(C5转化酶)。凝集素途径由甘露糖结合凝集素(MBL)或ficolin识别病原体表面糖
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