- ￥5300
- GeneTex
- 美国
- GTX50033
- 2025年07月07日
- WB, IHC-P, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Wnt3a (19-352aa) purified from E. coli
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX50033
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IHC-P, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Wnt3a
- 抗体英文名：
Wnt3a antibody [3A6]
- 抗体名：
Wnt3a 抗体 [3A6]
- 规格：
100 μl
WB analysis of various sample lysates using GTX50033 Wnt3a antibody [3A6].
Lane 1 : 293T whole cell lysate
Lane 2 : Wnt3a transfected 293T cell lysate
Dilution : 1:1000
Loading : 40μg per lane
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
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文献和实验Systemor equivalent). 4. Nitrocellulose or polyvinyl difluoride (PVDF) membrane. 5. Wnt3A antibody (available from R&D Systems, Minneapolis,MN). 6. Appropriate conjugated secondary antibody for detection. 2.4.2. b-Catenin Stabilization
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
cellular GSK3 and assay asabove, although this may be a very transient effect as we(Fig. 4.3 ) and others (10 , 12) do not see any change in GSK3specific activity beyond 30 min exposure of cells to Wnt. Fig. 4.1. Wnt3a regulates GSK3-mediated
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