- ￥5300
- GeneTex
- 美国
- GTX88003
- 2025年07月11日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Histone H4K12ac (8-59).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX88003
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H4K12ac (acetyl Lys12)
- 抗体英文名：
Histone H4K12ac (acetyl Lys12) antibody
- 抗体名：
Histone H4K12ac (acetyl Lys12) 抗体
- 规格：
100 μg
ICC/IF analysis of HeLa cells using GTX88003 Histone H4K12ac (acetyl Lys12) antibody. The picture on the right is blocked with the synthesized peptide.
WB analysis of COS7 cells treated with TSA 400nM (24h) lysate using GTX88003 Histone H4K12ac (acetyl Lys12) antibody. The lane on the right is blocked with the synthesized peptide.
IHC-P analysis of human breast carcinoma tissue using GTX88003 Histone H4K12ac (acetyl Lys12) antibody. The picture on the right is blocked with the synthesized peptide.
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文献和实验Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potential deacetylase enzymes
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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