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- 文献和实验
- 技术资料
- 免疫原:
Full-length protein expressed in 293T cell transfected with human TRPM4 expression vector
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83491
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IHC-P
- 浓度:
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
TRPM4
- 抗体英文名:
TRPM4 antibody [14C3]
- 抗体名:
TRPM4 抗体 [14C3]
- 规格:
100 μl
IHC-P analysis of liver carcinoma tissue using GTX83491 TRPM4 antibody [14C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of liver tissue using GTX83491 TRPM4 antibody [14C3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
WB analysis of PC12 cell lysate using GTX83491 TRPM4 antibody [14C3].
Loading : 10 ug per lane
Dilution : 1:200
WB analysis of HEK293T cells transfected with TRPM4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83491 TRPM4 antibody [14C3].
Loading : 5 ug per lane
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文献和实验Making antibodies to synthetic peptides
You'll need to couple the synthetic peptide hapten to a protein carrier, with a bifunctional reagent. For antibody development, we don't bother to use highly purified peptide. Crude (unpurified) peptide or partially purified peptide
Making antibodies to synthetic pepti
You'll need to couple the synthetic peptide hapten to a protein carrier, with a bifunctional reagent. For antibody development, we don't bother to use highly purified peptide. Crude (unpurified) peptide or partially purified peptide
a very good article,hope to study together with you!:) ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION INTRODUCTION Immunoprecipitation is a procedure by which peptides or proteins that react specifically with an antibody are removed
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