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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83588
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS
- 浓度:
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Sorbitol Dehydrogenase
- 抗体英文名:
Sorbitol Dehydrogenase antibody [1D5]
- 抗体名:
Sorbitol Dehydrogenase 抗体 [1D5]
- 规格:
100 μl
ICC/IF analysis of COS7 cells transiently transfected with Sorbitol Dehydrogenase plasmid using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
FACS analysis of HEK293T cells transfected with either Sorbitol Dehydrogenase plasmid(Red) or empty vector control plasmid(Blue) using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Dilution : 1:1000
FACS analysis of Jurkat cells using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Red : Primary antibody
Blue : Negative control antibody
ICC/IF analysis of HepG2 cells using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
FACS analysis of HeLa cells using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Red : Primary antibody
Blue : Negative control antibody
WB analysis of various cell lines using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Loading : 35 ug per lane
Dilution : 1:200
WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83588 Sorbitol Dehydrogenase antibody [1D5].
Loading : 5 ug per lane
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文献和实验Fernando E Garc?a-Arroyo et al., Oxid Med Cell Longev 2020 (PMID:33354281)
Garc?a-Arroyo FE et al., Int J Mol Sci 2019 (PMID:31744099)
Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Dehydrogenase (LDH) Measurement
In order to improve therapeutic antibodies efficacy in cancer patients, several strategies were developed. One of these strategies consists in the enhancement of effector functions. Antibody-dependent cellular cytotoxicity (ADCC) was shown
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
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