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- ￥7300
- GeneTex
- 美国
- GTX83922
- 2025年07月14日
- WB, ICC/IF, IHC-P, FACS
- Mouse
- Human
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- 文献和实验
- 技术资料
- 免疫原：
Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX83922
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS
- 浓度：
0.55 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PDE4A
- 抗体英文名：
PDE4A antibody [7B11]
- 抗体名：
PDE4A 抗体 [7B11]
- 规格：
100 μl
IHC-P analysis of human prostate tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of HEK293T cells transfected with either PDE4A plasmid(Red) or empty vector control plasmid(Blue) using GTX83922 PDE4A antibody [7B11].
IHC-P analysis of human endometrium tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human kidney tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
WB analysis of HEK293T cells transfected with PDE4A plasmid (Right) or empty vector (Left) for 48 hrs using GTX83922 PDE4A antibody [7B11].
Loading : 5 ug per lane
ICC/IF analysis of COS7 cells transiently transfected with PDE4A plasmid using GTX83922 PDE4A antibody [7B11].
IHC-P analysis of human colon tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
ICC/IF analysis of HT29 cells using GTX83922 PDE4A antibody [7B11].
WB analysis of various cell lines using GTX83922 PDE4A antibody [7B11].
Loading : 10 ug per lane
Dilution : 1:200
IHC-P analysis of human prostate carcinoma tissue using GTX83922 PDE4A antibody [7B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of Jurkat cells using GTX83922 PDE4A antibody [7B11].
Red : Primary antibody
Blue : Negative control antibody
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Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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