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![SEK1 / MKK4 antibody [4B4]](https://img1.dxycdn.com/2023/0711/451/6106825198874858761-14.jpg)
SEK1 / MKK4 ant
ibody [4B4]Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector
IgG2a
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Monoclonal
Unconjugated
Human
12 months from the shipping date of the product.
Human
GTX84161
Primary Antibodies
Available
GeneTex
Mouse
WB, ICC/IF, IHC-P, FACS, IP
1.09 mg/ml (Please refer to the vial label for the specific concentration.)
SEK1 / MKK4
SEK1 / MKK4 antibody [4B4]
SEK1 / MKK4 抗体 [4B4]
100 μl
ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].
IHC-P analysis of human kidney tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human colon tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human breast tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84161 SEK1 / MKK4 antibody [4B4].
Loading : 5 ug per lane
IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84161 SEK1 / MKK4 antibody [4B4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84161 SEK1 / MKK4 antibody [4B4].
IHC-P analysis of human lung tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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