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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat, Dog
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX84498
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Fumarate hydratase
- 抗体英文名:
Fumarate hydratase antibody [9G4]
- 抗体名:
Fumarate hydratase 抗体 [9G4]
- 规格:
100 μl
ICC/IF analysis of COS7 cells transiently transfected with Fumarate hydratase plasmid using GTX84498 Fumarate hydratase antibody [9G4].
WB analysis of HEK293T cells transfected with Fumarate hydratase plasmid (Right) or empty vector (Left) for 48 hrs using GTX84498 Fumarate hydratase antibody [9G4].
Loading : 5 ug per lane
IHC-P analysis of human breast tissue using GTX84498 Fumarate hydratase antibody [9G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
FACS analysis of HEK293T cells transfected with either Fumarate hydratase plasmid(Red) or empty vector control plasmid(Blue) using GTX84498 Fumarate hydratase antibody [9G4].
IHC-P analysis of human endometrium tissue using GTX84498 Fumarate hydratase antibody [9G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
WB analysis of various cell lines using GTX84498 Fumarate hydratase antibody [9G4].
Loading : 35 ug per lane
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文献和实验延胡索酸水合酶 fumarate hydratase 亦称延胡索酸酶。是催化延胡索酸 H2 O L-苹果酸可逆转变的酶。 EC4. 2. 1. 2。最适 pH为 6.5— 8.0。对底物有严格的特异性,反应平衡稍向右偏。在生物界广泛分布,可从肝脏、心肌和骨骼肌中得到纯品,从心肌得到的结晶品分子量约为 20万,由 4个多肽亚基组成。为柠檬酸循环中的酶,所以存在于线粒体的衬质中。
Mol Cell:南京医科大学钱旭团队揭示延胡索酸促进肿瘤恶化新机制
的 Fumarate 通过半胱氨酸琥珀酸化修饰 PTEN,抑制 PTEN 生物学活性,进一步促进肿瘤恶性进程。 延胡索酸(Fumarate)是三羧酸循环的重要中间代谢产物,能够通过延胡索酸水合酶(Fumarate Hydratase, FH)生成苹果酸,有研究表明 FH 在多种肿瘤中常发生突变,而 FH 的失活突变直接导致肿瘤细胞内 Fumarate 累积,促进肿瘤的发生发展。已有报道表明 Fumarate 做为 α-酮戊二酸(α-KG)的类似物,能够介导表观遗传重编程,其通过竞争性抑制 α-KG 依赖的组蛋白
case, the antibody should be added in 1:100 to 1:500 final dilution. • For Hsp70, 3A3 and C92 did work, 4G4 and 5A5 did not. 3A3 may be used 1 μl/ sample. • HSE probe must have high-specific activity (>10,000 cpm/fmol) and be double-stranded
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