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![alpha B Crystallin antibody [6D11]](https://img1.dxycdn.com/2023/0711/451/6106825198874858761-14.jpg)
alpha B Crystal
lin antibody [6D11]Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
IgG1
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Monoclonal
Unconjugated
Human, Rat, Monkey
12 months from the shipping date of the product.
Human
GTX84657
Primary Antibodies
Available
GeneTex
Mouse
WB, ICC/IF, IHC-P, FACS, IP
1 mg/ml (Please refer to the vial label for the specific concentration.)
alpha B Crystallin
alpha B Crystallin antibody [6D11]
alpha B Crystallin 抗体 [6D11]
100 μl
WB analysis of various cell lines using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 35 ug per lane
ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].
WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 5 ug per lane
WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84657 alpha B Crystallin antibody [6D11].
Loading : 10 ug per lane
Dilution : 1:200
IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84657 alpha B Crystallin antibody [6D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
IHC-P analysis of human thyroid carcinoma tissue using GTX84657 alpha B Crystallin antibody [6D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84657 alpha B Crystallin antibody [6D11].
ICC/IF analysis of HT29 cells using GTX84657 alpha B Crystallin antibody [6D11].
IHC-P analysis of human smooth muscle cancer tissue using GTX84657 alpha B Crystallin antibody [6D11].
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