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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Dog, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX84685
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS
- 浓度:
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Chk2
- 抗体英文名:
Chk2 antibody [2F4]
- 抗体名:
Chk2 抗体 [2F4]
- 规格:
100 μl
FACS analysis of HEK293T cells transfected with either Chk2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84685 Chk2 antibody [2F4].
WB analysis of various cell lines using GTX84685 Chk2 antibody [2F4].
Loading : 35 ug per lane
WB analysis of various samples using GTX84685 Chk2 antibody [2F4].
Loading : 10 ug per lane
Dilution : 1:200
ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84685 Chk2 antibody [2F4].
WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84685 Chk2 antibody [2F4].
Loading : 5 ug per lane
ICC/IF analysis of HT29 cells using GTX84685 Chk2 antibody [2F4].
WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX84685 Chk2 antibody [2F4].
Loading : 10 ug per lane
Dilution : 1:200
WB analysis of wild type and Chk2 knock out HeLa cell lysate(10μg per lane) using GTX84685 Chk2 antibody [2F4].
Dilution : 1:500
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文献和实验checkpoint regulator that responds to DNA damage. Because CHK1 and CHK2 share some substrates such as CDC25C in vitro, this assay could also be used for CHK2 activity assay, except that the CHK2 antibody will replace the CHK1 antibody.
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens matrix metalloproteinase-like 1 (MMPL1), mRNA 695 NM_001950 Homo sapiens E2F transcription factor 4, p107/p130-binding (E2F4), mRNA 956 NM_003815 Homo sapiens a disintegrin and metalloproteinase domain 15 (metargidin) (ADAM15), mRNA
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