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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX84937
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Adenylate kinase 1
- 抗体英文名:
Adenylate kinase 1 antibody [18C7]
- 抗体名:
Adenylate kinase 1 抗体 [18C7]
- 规格:
100 μl
WB analysis of wild type and Adenylate kinase 1 knock out HeLa cell lysate(10μg per lane) using GTX84937 Adenylate kinase 1 antibody [18C7].
Dilution : 1:500
ICC/IF analysis of HeLa cells transiently transfected with Adenylate kinase 1 plasmid using GTX84937 Adenylate kinase 1 antibody [18C7].
FACS analysis of HEK293T cells transfected with either Adenylate kinase 1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84937 Adenylate kinase 1 antibody [18C7].
WB analysis of HEK293T cells transfected with Adenylate kinase 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84937 Adenylate kinase 1 antibody [18C7].
Loading : 5 ug per lane
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
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