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![HDAC4 antibody [N3C1], Internal](https://img1.dxycdn.com/2023/0711/255/2344116916935858761-14.jpg)
HDAC4 antibody
[N3C1], InternalRecombinant protein encompassing a sequence within the center region of human HDAC4. The exact sequence is proprietary.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat
12 months from the shipping date of the product.
Human
GTX110231
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IHC-Fr, IP
2.1 mg/ml (Please refer to the vial label for the specific concentration.)
HDAC4
HDAC4 antibody [N3C1], Internal
HDAC4 抗体 [N3C1], Internal
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4(GTX110231) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed HeLa, using HDAC4(GTX110231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal (GTX110231)
5 % SDS-PAGE
The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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