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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant protein A from Staphylococcus aureus.
- 亚型:
IgY
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Biotin
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX77597
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Chicken
- 应用范围:
WB, ELISA
- 浓度:
0.56 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Protein A
- 抗体英文名:
Protein A antibody (Biotin)
- 抗体名:
Protein A 抗体 (Biotin)
- 规格:
1 ml
Indirect ELISA analysis performed by coating plate with recombinant full-length Protein A protein (0.2 μg/mL). Coated protein probed with Protein A antibody (Biotin) (GTX127324-02) (14-10000 pg/mL). The peroxidase streptavidin secondary antibody (1:10000) detected bound primary antibody.
Protein A antibody detects Protein A protein by western blot analysis. Whole cell extracts (0.1ng and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Protein A antibody (GTX77597) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Protein Carbonyl Determination Using Biotin Hydrazide
Protein oxidation is a recognized component of aging and a consequence of severe or prolonged exposure to reactive oxygen species (ROS). Direct attack of protein by ROS causes formation of protein-bound carbonyl groups (1 ). These carbonyl
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
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