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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Pooled rat serum IgG
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX76234
- 级别:
Secondary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
FACS
- 浓度:
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Recognizes a determinant on rat kappa chains and binds preferentially to the Ig-1b allotype.
- 抗体英文名:
Mouse Anti-Rat kappa Light chain antibody [MRC OX-12]
- 抗体名:
Mouse Anti-Rat kappa Light chain 抗体 [MRC OX-12]
- 规格:
250 μg
Staining of rat splenic lymphocytes with Mouse anti Rat kappa light chains (GTX76234)
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文献和实验Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
information for VH and VL is generally amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody-specific primers A variety of primer sets for the amplification of mouse-variable domams has been developed that are particulary
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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