- ￥6600
- GeneTex
- 美国
- GTX75792
- 2025年07月14日
- WB, FACS, IP, Functional Assay
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peripheral blood mononuclear cells.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX75792
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS, IP, Functional Assay
- 浓度：
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells.
- 抗体英文名：
CD18 antibody [MEM-148] (Azide free)
- 抗体名：
CD18 抗体 [MEM-148] (Azide free)
- 规格：
500 μg
FACS analysis of human peripheral blood granulocytes using GTX75792 CD18 antibody [MEM-148] (Azide free).
FACS analysis of human peripheral blood monocytes using GTX75792 CD18 antibody [MEM-148] (Azide free).
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文献和实验Li N et al., J Immunol 2013 (PMID:23514737)
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD18
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