- ￥6500
- GeneTex
- 美国
- GTX75485
- 2025年07月08日
- WB, FACS, IP
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX75485
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domai
- 抗体英文名：
CD147 antibody [MEM-M6/1] (Azide free)
- 抗体名：
CD147 抗体 [MEM-M6/1] (Azide free)
- 规格：
500 μg
WB analysis of HepG2 cell lysate using GTX75485 CD147 antibody [MEM-M6/1] (Azide free).
FACS analysis of human erythrocytes using GTX75485 CD147 antibody [MEM-M6/1] (Azide free).
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文献和实验Kornek M et al., Hepatology 2011 (PMID:20979056)
Nikas IP et al., Cancer Med 2022 (PMID:35212471)
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Phycoerythrin conjugation protocol
. I used 117 x 1 cm. You can't really get away with anything smaller. Use a long thin one rather than a short fat one, since the latter did not work for me. Separation of the conjugate from the free reagents is tricky. *
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