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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
T-end.1, a pMT transformed endothelial cell line.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
FITC
- 适应物种:
Mouse, Rabbit
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX75412
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
ICC/IF, FACS, IHC
- 浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody recognizes a neuraminidase sensitive epitope.
- 抗体英文名:
CD34 antibody [MEC14.7] (FITC)
- 抗体名:
CD34 抗体 [MEC14.7] (FITC)
- 规格:
100 μg
FACS analysis of mouse bone marrow cells using GTX75412 CD34 antibody [MEC14.7] (FITC).
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文献和实验Urbani L et al., PLoS One 2012 (PMID:23166781)
Peng YJ et al., J Biomed Mater Res A 2012 (PMID:22337602)
当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
ml三蒸水中即成; 方法与步骤: 根据Marshall氏法高效价的抗人球蛋白兔免疫血清,分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg,缓冲液为总量的10%; 2. 将以上溶液降温至4℃,按蛋白:荧光素=50—80mg:1mg的比例加入异硫氰酸荧光素,在0—4℃下电磁搅拌12—14h; 3.用半饱和硫酸铵将标记球蛋白
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