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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Normal human peripheral blood lymphocytes.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Human, Chimpanzee, Cynomolgus monkey, Marmoset, Red-bellied Tamarin
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX74777
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
FACS
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
CD8
- 抗体英文名:
CD8 antibody [LT8] (PE)
- 抗体名:
CD8 抗体 [LT8] (PE)
- 规格:
100 test
Figure A. Alexa647R conjugated rat anti human CD3 and RPE conjugated Mouse IgG1 isotype control (GTX76639). Figure B. Alexa647R conjugated rat anti human CD3 and RPE conjugated mouse anti human CD8 (GTX74777). All experiments performed on red cell lysedand permeabilized Human peripheral blood gated on leukocytes
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文献和实验Huang CP et al., Cytotherapy 2017 (PMID:28818454)
Lask A et al., J Immunol 2011 (PMID:21753148)
Ma Q et al., Precision Clinical Medicine 2021;4(3)
Qizhi Ma et al., Precis Clin Med 2021 (PMID:35693216)
Chebloune Y et al., Vaccine 2020 (PMID:32278522)
Buchner SM et al., Clin Exp Immunol 2013 (PMID:23607364)
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
体,实现了对抗原特异性 (CD8+ T) 细胞的检测和分离 (如,流式分析) 。此技术的特点在于,荧光或磁性微珠等标记物可以通过使用 Biotin (生物素) 而去除,保留了细胞的真实特性,得到的功能性的,label-free 的细胞可用于下游的实验研究。 1) MHC I STREPTAMERS® 技术解析 MHC I STREPTAMERS® 由两部分组成: · MHC I-Strep,即连接了 Twin-Strep-tag® 和抗原肽的 MHC I 分子。 · 荧光 (PE/APC)
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