- ￥6600
- GeneTex
- 美国
- GTX72682
- 2025年07月10日
- IHC-P, FACS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Small cell lung carcinoma cells
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX72682
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
IHC-P, FACS
- 靶点：
EpCAM
- 抗体英文名：
EpCAM antibody [VU-1D9] (Biotin)
- 抗体名：
EpCAM 抗体 [VU-1D9] (Biotin)
- 规格：
100 μl
IHC-P analysis of human breast carcinoma tissue using GTX72682 EpCAM antibody [VU-1D9] (Biotin).
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文献和实验Lyberopoulou A et al., PLoS One 2015 (PMID:25902072)
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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