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- 文献和实验
- 技术资料
- 免疫原:
Amino acids 477-947 of human SRC-1.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX70250
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IP, ChIP assay
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SRC1
- 抗体英文名:
SRC1 antibody [1135]
- 抗体名:
SRC1 抗体 [1135]
- 规格:
100 μl
Mouse tissue (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] (GTX70250) diluted at 1:2000.
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文献和实验Dutertre M et al., Mol Endocrinol 2003 (PMID:12714702)
Amita M et al., Endocrinology 2010 (PMID:19934375)
Jaber BM et al., Mol Endocrinol 2006 (PMID:16840538)
Klinge CM et al., J Mol Endocrinol 2004 (PMID:15525597)
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Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1](E-AB-F0995Q)、PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1](E-AB-F0994H)、APC Anti-Mouse CD206/MMR Antibody[C068C2](E-AB-F1135E)染色并使用流式细胞仪检测分析巨噬细胞的功能分型结果。CD11bhi/midF4/80hi/mid巨噬细胞,为炎性活化的巨噬细胞,进一步分析该细胞群中CD206和CD
Metabolic Labeling and Immunoprecipitation of Yeast Proteins
Detergent IP buffer (see recipe ) Antibody specific for protein of interest Preimmune serum
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16/32 Antibody,轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer轻轻吹打混匀,150 × g离心3 min,弃上清; (6)加入200 μL Cell Staining Buffer重悬细胞沉淀,上机检测。 图1. Raw264.7细胞极化培养流程 2. Raw264.7细胞向M1型巨噬细胞极化结果 图
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