- ￥4700
- GeneTex
- 美国
- GTX11997
- 2025年07月12日
- WB, ICC/IF, IHC-P, IHC-Fr
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
This antibody was developed against a peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. In mouse, alanine at position 8 is replaced by methionine.
- 亚型：
IgG1, Kappa
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX11997
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
p73 delta N
- 抗体英文名：
p73 delta N antibody [38C674.2]
- 抗体名：
p73 delta N 抗体 [38C674.2]
- 规格：
50 μg
IHC-P analysis of human lung squamous cell carcinoma tissue using GTX11997 p73 delta N antibody [38C674.2].
Dilution : 5 μg/ml
ICC/IF analysis of HeLa cells using GTX11997 p73 delta N antibody [38C674.2].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:50
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100
WB analysis of (A) p73 delta N overexpressing (B) HeLa, and (C) NIH-3T3 cell lysate using GTX11997 p73 delta N antibody [38C674.2].
Dilution : 1 μg/ml
IHC-P analysis of human placenta tissue using GTX11997 p73 delta N antibody [38C674.2].
Dilution : 5 μg/ml
Antigen retrieval : Heat-induced antigen retrieval
IHC-Fr analysis of E15 (A) and E17 (B) rat embryonic kidney tissue using GTX11997 p73 delta N antibody [38C674.2]. Delta Np73 is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the glomerular cleft. DeltaNp73 is expressed in the ureteric bud (UB) branches, metanephrogenic mesenchyme (MM), and nephron progenitors.
Dilution : 1:2000
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
自然染色质免疫沉淀实验设计（Native Chromatin Immunoprecipitation (N-ChIP)）
to obtain a long/medium oligonucleosome ladder. If pure mononucleosome preparations are required carry out a linear sucrose gradient (5-20%),this will increase resolution. 2.1. Perform microccal nuclease digestions at 37°C for 5 min. 2.2. Stop
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