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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A bacterially expressed, mouse CHOP fusion protein.
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Rabbit
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX11419
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP
- 抗体英文名:
GADD153 antibody [9C8]
- 抗体名:
GADD153 抗体 [9C8]
- 规格:
50 μg
WB analysis of 30 ug of whole cell lysates from undifferentiated 3T3-L1 fibroblasts (Fib) and 3T3-L1 fibroblasts differentiated into adipocytes (Day 2 and Day 9 post-differentiation) using GTX11419 GADD153 antibody [9C8].
3T3-L1 fibroblasts were cultured in differentiation medium for 3 days to generate adipocytes. Differentiated adipocytes were maintained in maturation media containing either 5mM glucose (normal glucose) or 30mM glucose (high glucose).
Dilution : 1:1000
WB analysis of HeLa cells were left untreated (-) or treated with tunicamycin (5 μg/ml) for 11 hours (+) using GTX11419 GADD153 antibody [9C8].
Dilution : 1:2000
ICC/IF analysis of A549 cells treated with media only (non-treated) or with 1.5 μM tunicamycin for 6 hours using GTX11419 GADD153 antibody [9C8].
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文献和实验Guan P et al., Life Sci 2019 (PMID:30951745)
Su CC et al., Anticancer Res 2014 (PMID:25503137)
Monceaux K et al., Int J Mol Sci 2022 (PMID:35743074)
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Protein A Purification of Antibody
Reagents Affi-gel Protein-A Agarose (BioRad #153-6153) MAPS II Binding Buffer (BioRad # 153-6161) 0.314 g/ml diH2 O MAPS II Elution Buffer (BioRad #153-6162)
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: 揭示靶向 NS1 的黄病毒广谱保护性抗体作用机制 2021 年 1 月 8 日,中国科学院微生物研究所 / 中国科学院北京生命科学研究院高福院士团队、中国科学院微生物所施一团队与澳大利亚昆士兰大学的 Paul R. Young 及 Daniel Watterson 团队联合在 Science 杂志发表了论文 A broadly protective antibody that targets the flavivirus NS1 protein, 研究结果发现了一株对多种黄病毒(登革病毒、西尼
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