- ￥5000
- GeneTex
- 美国
- GTX10438
- 2025年07月16日
- WB, IP, ELISA
- Mouse
- Human, Mouse, Rat, Bovine, Chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Bovine, Chicken
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX10438
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IP, ELISA
- 靶点：
AKT1
- 抗体英文名：
AKT1 antibody [PKB-175]
- 抗体名：
AKT1 抗体 [PKB-175]
- 规格：
50 μl
WB analysis of (1)HeLa (2) HepG2 (3) A549 (4) HEK-293 (5) JURKAT (6) MCF-7 cells using GTX10438 AKT1 antibody [PKB-175] at 1:5,000.
IP analysis of MCF-7 cells using GTX10438 AKT1 antibody [PKB-175].
Lane 1 : IP from MCF-7 cells using10 μL antibody
Lane 2 : IP from MCF-7 cells using10 μL nonrelevant antibody
Lane 3 : MCF-7 whole cell lysate
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文献和实验相关专题 细胞凋亡专题 PATHWAYS KEY: Akt也被称为蛋白激酶B(protein kinase B，PKB)，是一种分子 量约为60kDa的丝/苏氨酸蛋白激酶，处于多条信号通路的重要交叉点，可调节细胞因子、生长因子和癌基因Ras激活的细胞生存信号，在真核生物的调控网络中普遍存在。Akt信号通路与恶性肿瘤、糖尿病、类风湿关节炎等多种疾病的发生发展密切
Assaying AKT/Protein Kinase B Activity
, or transforming oncogenes ( 2 ). These agonists all stimulate Akt/PKB by promoting its phosphorylation on two regulatory residues (e.g., S473 and T308 for the Akt1 isoform), an event dependent on the prior activation of a signaling pathway initiated
相关专题 Akt1, also known as "Akt" or protein kinase B (PKB). Its signalling pathway is as follows: 1. Binds to phospholipids in the membrane 2. Phosphorylation by PDK1 and PDK2 3. Lipid phosphatases
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