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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein. [Swiss-Prot# Q13148]
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.
- 克隆性:
Polyclonal
- 标记物:
DyLight550
- 适应物种:
Human, Mouse, Zebrafish, Chicken, Xenopus, Primate
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX48595
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
TDP43
- 抗体英文名:
TDP43 antibody (DyLight 550)
- 抗体名:
TDP43 抗体 (DyLight 550)
- 规格:
50 μl
ICC/IF analysis of human iPS derived neuron cells using GTX48595 TDP43 antibody (DyLight 550).
Red : primary antibody
Blue : DAPI
Dilution : 1:200
Permibilization : 0.3% Triton X-100
Fixation : 4% PFA
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文献和实验人脑神经相关生长蛋白-43(GAP-43)ELISA试剂盒 说明书
人脑神经相关生长蛋白-43 ( GAP-43 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 GAP-43 单抗包被于酶标板上,标准品和样品中的 GAP-43与单抗结合,加入生物素化的抗人 GAP-43 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,GAP-43
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid
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