- ￥6600
- GeneTex
- 美国
- GTX40574
- 2025年07月07日
- WB, Dot, ELISA, IM
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carboxypeptidase Y (S. cerevisiae (Baker's Yeast)).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
HRP
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX40574
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, Dot, ELISA, IM
- 浓度：
10 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y (Baker's Yeast).
- 抗体英文名：
Carboxypeptidase Y antibody (HRP)
- 抗体名：
Carboxypeptidase Y 抗体 (HRP)
- 规格：
1 mg
Sheep anti Lactoperoxidase antibody (GTX40571) was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in blocking buffer). Detection shown was using Dylight 488 conjugated secondary antibody (1:10K in TBS/blocking buffer 1 hr RT).
Western Blot of Peroxidase conjugated Rabbit anti-Carboxypeptidase Y Antibody (GTX40574). Lane 1: Carboxypeptidase Y. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit Carboxypeptidase Y antibody at 1:1,000 for 60 min at RT Block for 30 min at RT. Predicted/Observed size: 53 kDa for Carboxypeptidase Y. Other band(s): none.
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文献和实验Preparation of Carboxypeptidase Y and Its Properties
while high efficiency habit in the scientific research. 【Requirement】 1. Use bread yeast as the original material to extract and condense carboxypeptidase Y, assay its biochemical properties. 2. Through gathering relevant published papers
Carboxypeptidase Y (EC 3.4.16.1)
sequence data. These are: carboxypeptidase A (EC 3.4.17-l) from bovine pancreas (1 ), carboxypeptidase B (EC 3.4.17.2) from porcine pancreas (1 ), carboxypeptidase C (EC 3.4.12.1) from orange leaves (2 ), and carboxypeptidase Y (EC 3.4.16.1) from yeast
Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
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