Aldolase A antibody (HRP)

Anti aldolase antibody (GTX40552) – Immunoprecipitation and Western Blot. 300 μl aliquots of whole anti-aldolase antiserum (GTX48869) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 μl antiserum with no antigen (negative control) 3. 300 μl antiserum with ~100 μl aldolase (2.5 mg/ml) 4. 300 μl antiserum with ~200 μl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4ºC, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH.  90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.  Gel was stained, destained and imaged using standard protocols.  Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase.  Additional higher and lower molecular weight bands correspond to serum proteins.

IgG purified antibody to rabbit muscle aldolase (GTX40552) was used at a 1:1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4º C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.