- ￥5300
- GeneTex
- 美国
- GTX24194
- 2025年07月16日
- WB, ICC/IF, ELISA
- Rabbit
- Human, Chimpanzee
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide - amino acids 2604-2614 of human DNA PKcs - phosphorylated at the pT2609 residue
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Chimpanzee
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24194
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNA-PKcs (phospho Thr2609)
- 抗体英文名：
DNA-PKcs (phospho Thr2609) antibody
- 抗体名：
DNA-PKcs (phospho Thr2609) 抗体
- 规格：
50 μg
Western blot using GeneTex Affinity Purified anti-DNAPKcs antibody (GTX24194) shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 μg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
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文献和实验Jarboe JS et al., Radiother Oncol 2012 (PMID:22561027)
Nowsheen S et al., PLoS One 2011 (PMID:21912620)
Jarboe JS et al., Clin Cancer Res 2012 (PMID:22619307)
Nixon BR et al., FASEB J 2018 (PMID:30106602)
Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage
a significant level of antibodies specific to the nonphosphorylated peptide is present in the antisera, an enhancement step is used to obtain a useful phospho-specific antibody. Although these enhanced antisera are suitable for many applications
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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