- ￥5300
- GeneTex
- 美国
- GTX24194
- 2025年07月16日
- WB, ICC/IF, ELISA
- Rabbit
- Human, Chimpanzee
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide - amino acids 2604-2614 of human DNA PKcs - phosphorylated at the pT2609 residue
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Chimpanzee
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24194
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNA-PKcs (phospho Thr2609)
- 抗体英文名：
DNA-PKcs (phospho Thr2609) antibody
- 抗体名：
DNA-PKcs (phospho Thr2609) 抗体
- 规格：
50 μg
Western blot using GeneTex Affinity Purified anti-DNAPKcs antibody (GTX24194) shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 μg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
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文献和实验Jarboe JS et al., Radiother Oncol 2012 (PMID:22561027)
Nowsheen S et al., PLoS One 2011 (PMID:21912620)
Jarboe JS et al., Clin Cancer Res 2012 (PMID:22619307)
Nixon BR et al., FASEB J 2018 (PMID:30106602)
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