- ￥5900
- GeneTex
- 美国
- GTX30465
- 2025年07月10日
- WB, ICC/IF, IHC-Fr, IP, IHC
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rat
- 目录编号：
GTX30465
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-Fr, IP, IHC
- 靶点：
This antibody is specific for STEP61, STEP46, STEP38, and STEP20.
- 抗体英文名：
STEP / PTPN5 antibody [23E5]
- 抗体名：
STEP / PTPN5 抗体 [23E5]
- 规格：
100 μl
WB analysis of rat striatal protein homogenates using GTX30465 STEP antibody [23E5].
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文献和实验step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
, and incubate for 1 hour on ice. Pellet cells, giving an antibody supernatant. Add this to a cell pellet prepared as in step 9. Be very careful not to get supernatants mixed up! Repeat steps 9 + 10 seven times, or as often as necessary to decrease
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