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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Hamster
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX30123
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
- 抗体英文名:
HIF2 alpha antibody [ep190b]
- 抗体名:
HIF2 alpha 抗体 [ep190b]
- 规格:
100 μl
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1: normoxic A549 lysate control
Lane 2: hypoxic A549 lysate
WB analysis of multiple samples using GTX30123 HIF2 alpha antibody [ep190b].
Lane 1 and 5: HepG2 without Cobalt (II) Chloride
Lane 2 and 6: HepG2 with Cobalt (II) Chloride
Lane 3 and 7: HepG2 normoxic
Lane 4 and 8 : HepG2 hypoxic
Dilution : 1-2 μg/ml
FACS analysis of HepG2 cells using GTX30123 HIF2 alpha antibody [ep190b]. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400
WB analysis of HeLa cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
IHC-P analysis of human heart tissue using GTX30123 HIF2 alpha antibody [ep190b].
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文献和实验Chen S et al., Angiogenesis 2010 (PMID:20221685)
Wright TM et al., J Biol Chem 2010 (PMID:20185829)
Myers K et al., PLoS Genet 2009 (PMID:19119425)
Hacker KE et al., PLoS One 2008 (PMID:19030229)
Nagalakshmi VK et al., Clin Sci (Lond) 2018 (PMID:30442812)
Zera K et al., PLoS One 2017 (PMID:29045486)
Catrina SB et al., Clin Cancer Res 2006 (PMID:16899596)
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
marrow. The prime suspect: an adhesion molecule called alpha2 integrin. The molecule showed increased expression on memory cells, and its ligand is predominantly expressed in bone marrow tissue. Also, when the researchers blocked alpha2 integrin
电泳(electrophoresis)是指带电荷的溶质或粒子在电场中向着与其本身所带电荷相反的电极移动的现象。利用电泳现象将多组分物质分离、分析的技术叫做电泳技术。可以实现电泳分离技术的 仪器 称之为电泳仪(electrophoresister)。电泳技术是由瑞典科学家A.Tiselius在1937年首先利用U形管建立了移界电泳法(moving boundary EP),即区带电泳( zone electrophoresis ),他成功地将血清
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