- ￥5300
- GeneTex
- 美国
- GTX29465
- 2025年07月14日
- ICC/IF, IHC-P, IHC-Fr, IHC-Wm
- Mouse
- Human, Mouse, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified rabbit skeletal α-actinin.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rabbit
- 目录编号：
GTX29465
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, IHC-P, IHC-Fr, IHC-Wm
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody is specific for α-skeletal muscle actinin and α-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.
- 抗体英文名：
sarcomeric alpha Actinin antibody [EA-53]
- 抗体名：
sarcomeric alpha Actinin 抗体 [EA-53]
- 规格：
500 μl
IHC-P analysis of human skeletal muscle tissue using GTX29465 sarcomeric alpha Actinin antibody [EA-53].
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文献和实验Milan M et al., Cell Death Dis 2018 (PMID:29371598)
Gong R et al., Pharmacol Res 2021 (PMID:34428587)
Williams AL et al., Physiol Genomics 2018 (PMID:29652636)
Bellmann J et al., Biomaterials 2019 (PMID:31614290)
) for a detailed hot-stop PCR protocol. Figure 1 provides an example of results using SSCP. To quantify how much DNA has precipitated at a locus of interest, we use real-time PCR (Steps 38-39) and duplex PCR (Steps 40-53). Examples of duplex PCR analysis
Introduction of human 14-3-3 proteins
terminus of GPIb-alpha,as indicated by antibody inhibition and direct binding of 14-3-3zeta to synthetic GPIb-alpha cytoplasmic domain peptides.The 14-3-3zeta binds to recombinant wild type GPIb-IX but not to the GPIb-alpha mutants lacking C-terminal
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
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