Standard PLGA nanoparticles, 1

Standard Ligation

. coli strain . Solutions: 10x Ligation Buffer: 0.5 M Tris-HCl pH 7.8, 50 mM MgCl2 , 0.1 M b-mercaptoethanol, 50 mM ATP, 5 mg/ml BSA  

Standard PCR Protocol

DNA markers going from 2kb down to 100 bp or less (recommend BM PCR markers).  View on UV light box at 254 - 300 nm, photo 1 - 5 sec. NOTE: Small products are best seen on 3% agarose gels that have been run fast (eg: 100 volts), with BPB run to ½

Standard Ligation

-mercaptoethanol, 50 mM ATP, 5 mg/ml BSA   Ligation Buffer (10