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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
Pipettes and Pipette Tips: 10 µL - 20 µL or 20 µL - 100 µL Multi-Channel Pipettes and Pipette Tips: 5 ~ 50 µL and 50 ~ 300 µL Buffer and Reagent Reservoirs Vortex Mixer Deionized Water Microtiter Plate Reader capable
NaCl 600mM NaCl 100mM Tris-HCl, pH 7.6 100mM Tris-HCl, pH 7.6 100mM MgCl2 70mM MgCl2 10mM DTT 10x High salt buffer 10x Sma I buffer (1) 1.0M NaCl 200mM KCl 500mM Tris-HCl, pH 7.6 100mM Tris-HCl, pH 7.6 100mM MgCl
BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
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