- ￥5000
- GeneTex
- 美国
- GTX27758
- 2025年07月11日
- ICC/IF, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4℃. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX27758
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
HLA-G
- 抗体英文名：
HLA-G antibody [MEM-G/9]
- 抗体名：
HLA-G 抗体 [MEM-G/9]
- 规格：
100 μg
Sandwich ELISA analysis of HLA-G and Beta 2-microglobulin in supernatant of HLA-G transfectants using GTX27758 HLA-G antibody [MEM-G/9] as capture antibody and GTX78317 beta 2 Microglobulin antibody [B2M-01] (HRP) as detection antibody.
Sandwich ELISA analysis of human serum using GTX27758 HLA-G antibody [MEM-G/9] as capture antibody and GTX78317 beta 2 Microglobulin antibody [B2M-01] (HRP) as detection antibody.
IP analysis of HLA-G1 transfectants using GTX27758 HLA-G antibody [MEM-G/9]. The signal was detected by GTX80268 HLA-G antibody [4H84] in WB assay.
Lane 1 : cell lysate
Lane 2 : HLA-G immunoprecipitate from cell lysate
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文献和实验Lin YC et al., iScience 2023 (PMID:36876120)
Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins
in our laboratory to distinguish mRNAs encoding HLA-G5 and HLA-G6, and antibody-based protocols for identification of the soluble isoforms.
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
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