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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX19066
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
WB, ICC/IF, IHC-P, ChIP assay
- 浓度:
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody is expected to recognize isoform 1 (NP_000035.2) only.
- 抗体英文名:
Androgen Receptor antibody, N-term
- 抗体名:
Androgen Receptor 抗体, N-term
- 规格:
100 μg
ICC/IF analysis of PFA fixed MCF7 cells using GTX19066 Androgen Receptor antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
WB analysis of human cerebellum (A) and negative control pancreas (B) lysate using GTX19066 Androgen Receptor antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
ICC/IF analysis of PFA fixed U2OS cells using GTX19066 Androgen Receptor antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
ChIP analysis of DHT-treated HEC50 cells using GTX19066 Androgen Receptor antibody, N-term. The precipitated DNA was detected by PCR with primer set targeting to FKBP5.
ChIP reaction : 2μg antibody / 1 μg chromatin
IHC-P analysis of human prostate using GTX19066 Androgen Receptor antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
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文献和实验Analysis of Androgen Receptor SUMOylation
Androgen receptor (AR) is a ligand-controlled transcription factor that is deregulated and therefore targeted in prostate cancer. In addition to androgens, AR is regulated by post-translational modifications (PTMs). SUMOylation, conjugation
Analysis of Interdomain Interactions of the Androgen Receptor
High-affinity binding of testosterone or dihydrotestosterone to the androgen receptor (AR) triggers the androgen-dependent AR NH2 - and carboxyl-terminal (N/C) interaction between the AR NH2 -terminal FXX LF motif and the activation function
In Vitro and In Vivo Silencing of the Androgen Receptor
The androgen receptor (AR) plays a pivotal role in the progression of prostate cancer from the androgen-dependent to the castration-resistant state, making it a potential target for therapy. In this chapter, we describe the preparation
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