- ￥4300
- GeneTex
- 美国
- GTX26662
- 2025年07月10日
- WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
- 克隆性：
Polyclonal
- 标记物：
FITC
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX26662
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Goat
- 应用范围：
WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
GFP
- 抗体英文名：
GFP antibody (FITC)
- 抗体名：
GFP 抗体 (FITC)
- 规格：
100 μg
Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.
Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
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文献和实验Mercapide J et al., Int J Cancer 2010 (PMID:19653275)
Evsen L et al., Development 2020 (PMID:32816902)
Laiouar S et al., Curr Biol 2020 (PMID:32059769)
Lale Evsen et al., bioRxiv 2019 (Epub)
Chan AH et al., PLoS One 2017 (PMID:28355300)
Clayton ZE et al., Biosci Rep 2018 (PMID:29976773)
Woichansky I et al., Nat Commun 2016 (PMID:26960923)
Raft S et al., Dev Biol 2014 (PMID:24583262)
Li X et al., PLoS Genet 2013 (PMID:23874234)
Chang W et al., PLoS Genet 2008 (PMID:18404215)
Raft S et al., PLoS One 2014 (PMID:25299585)
de Renty C et al., PLoS One 2014 (PMID:24848107)
Berns N et al., J Cell Sci 2014 (PMID:24762813)
Hwang CH et al., Dev Dyn 2009 (PMID:19842177)
Evsen L et al., J Neurosci 2013 (PMID:23447599)
Gomez JM et al., J Cell Biol 2012 (PMID:23266957)
Klausner EA et al., J Gene Med 2012 (PMID:22106057)
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
对照，2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R]（货号#E022030），其免疫原是KLH偶联的水母GFP蛋白的N末端多肽，由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致，因此从免疫原上来说，GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是，EarthOx对于该单克隆抗体还进行了明确的验证试验，试验也表明该Anti-GFP Tag Monoclonal
流式课堂 | 细胞带有 GFP 荧光时，如何进行流式凋亡分析？
、FITC、EGFP 等。 在这里，比较推荐的荧光组合是 Annexin-V APC/7AAD。APC 的激发/发射波长为 650/660，7AAD 的激发/发射波长为546/650，可以很好地降低 GFP 带来的干扰，使结果分析更加准确。 二、如何分析结果 在选择了合适的试剂盒进行上机检测后，恰当的结果分析，对于数据的完美呈现，也是至关重要的。以前文数据为例，我们来看看如何分析： 01 设置 FSC/SSC 圈门 根据大小及颗粒度，圈出需要分析的细胞群体，排除碎片及黏连体的影响； 02 调整荧
GFP抗体|GFP抗体检测GFP、EGFP、YFP、EYFP、CFP抗体
EarthOx GFP标签抗体（#E022030）用于WB实验，1道为阴性对照，2道为1:1000倍稀释 我们先看一下EarthOx的单克隆GFP抗体 ――Anti-GFP Tag Monoclonal Antibody [GF28R]（货号#E022030），其免疫原是KLH偶联的水母GFP蛋白的N末端多肽，由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致，因此从免疫原上来说，GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是，EarthOx对于该单克
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