- ￥5300
- GeneTex
- 美国
- GTX26577
- 2025年07月16日
- WB, IHC-P, FACS, IP, ELISA
- Rabbit
- Human, Mouse, Rat, Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Collagen Type I from human and bovine placenta.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Biotin
- 适应物种：
Human, Mouse, Rat, Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human|Bovine
- 目录编号：
GTX26577
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, FACS, IP, ELISA
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
Collagen I
- 抗体英文名：
Collagen I antibody (Biotin)
- 抗体名：
Collagen I 抗体 (Biotin)
- 规格：
50 μg
Western Blot of Rabbit anti-Collagen I antibody (GTX26577). Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 μg per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I antibody at 0.2–2 μg/10 ml for overnight at 4ºC. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 μg/10 ml for overnight at 4ºC. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.
Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody (GTX26577). Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B)
Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody (GTX26577). Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B)
Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody at 5μg/mL for 45 min at 4ºC. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT.
Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody. Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.
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The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
Collagen Antibody-Induced Arthritis: A Disease-Relevant Model for Studies of Persistent Joint Pain
is the collagen-induced arthritis (CIA) model. However, it has certain disadvantages when used for pain research. Here we describe an alternative model, the collagen antibody-induced arthritis (CAIA) model to study RA-induced pain. This model has been used
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