- ￥5600
- GeneTex
- 美国
- GTX25190
- 2025年07月15日
- WB, ICC/IF, FACS, IP
- Rabbit
- Human, Cat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin b3 receptor that contains tyrosine 773 (based on Swiss Protein database, accession number P05106).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Cat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25190
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, FACS, IP
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Integrin beta 3 (phospho Tyr773)
- 抗体英文名：
Integrin beta 3 (phospho Tyr773) antibody
- 抗体名：
Integrin beta 3 (phospho Tyr773) 抗体
- 规格：
50 μl
ICC/IF analysis of THP-1 cells using GTX25190 Integrin beta 3 (phospho Tyr773) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with GTX25190 Integrin beta 3 (phospho Tyr773) antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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dongreyuan 有的抗体名称上面写着Ab-33，比如，β-catenin（Ab-33）antibody，请问，Ab-33是什么意思？ bettyzyj 这个抗体是非磷酸化的抗体non-phospho-antibody ，33 代表的是基因位点 dongreyuan 谢谢了 yhwangcams bettyzyj wrote
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