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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide with sequence C-AAQVKAPLVLKD, from the C Terminus of the protein sequence according to NP_009193.2.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX24150
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Park7 / DJ-1
- 抗体英文名:
Park7 / DJ-1 antibody
- 抗体名:
Park7 / DJ-1 抗体
- 规格:
50 μg
IHC-P analysis of human thyroid using GTX24150 Park7 / DJ-1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
WB analysis of HeLa (A) and Jurkat (B) lysates using GTX24150 Park7 / DJ-1 antibody.
Dilution : 0.001μg/ml
Loading : 35μg protein in RIPA buffer
WB analysis of human cerebellum (A), human frontal cortex (B), human hippocampus (C) mouse Fetal brain (D), mouse brain (E) and rat brain (F) lysates using GTX24150 Park7 / DJ-1 antibody.
Dilution : 0.001μg/ml
Loading : 35μg protein in RIPA buffer
ICC/IF analysis of MCF7 (A) and HeLa (B) cells using GTX24150 Park7 / DJ-1 antibody.
Dilution : 5μg/ml
IHC-P analysis of human prostate using GTX24150 Park7 / DJ-1 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
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