- ￥5300
- GeneTex
- 美国
- GTX22286
- 2025年07月12日
- WB, ELISA
- Mouse
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX22286
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
- 抗体英文名：
Phosphothreonine antibody [18F6]
- 抗体名：
Phosphothreonine 抗体 [18F6]
- 规格：
50 μl
Mab anti-phosphothreonine antibody (clone 18F6, GTX22286) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1,000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using 1:2,500 dilution Goat-a-Mouse IgG [H&L] secondary antibody and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.
ELISA results of Mab anti-phosphothreonine antibody (GTX22286) tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1μg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) antibody and TMB substrate were used for detection.
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文献和实验-phosphoserine的抗体。 摘抄： Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner
Intracellular Immunofluorescent Staining for Flow Cytometry
Guide Mouse Cytokines: Intracellular Staining Quick Guide Mouse Cytokine Cell Source Activation Incubation Time Restimulation Intracellular Block Antibody
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